![SOLVED: For the following experiment; we want the His-Tag to be placed at the C-terminal end. Please add a restriction enzyme cut site to each of the original primers to that protein SOLVED: For the following experiment; we want the His-Tag to be placed at the C-terminal end. Please add a restriction enzyme cut site to each of the original primers to that protein](https://cdn.numerade.com/ask_images/9ba1accee44043f5a7c0785c83c65d99.jpg)
SOLVED: For the following experiment; we want the His-Tag to be placed at the C-terminal end. Please add a restriction enzyme cut site to each of the original primers to that protein
![Regarding cloning of Nus His tag fusion construct, to express double tagged fusion protein ? | ResearchGate Regarding cloning of Nus His tag fusion construct, to express double tagged fusion protein ? | ResearchGate](https://www.researchgate.net/profile/Monika-Jain-11/post/Regarding_cloning_of_Nus_His_tag_fusion_construct_to_express_double_tagged_fusion_protein/attachment/59de52a6b53d2f8eb259d2de/AS%3A548325414129664%401507742374498/download/pET28a.png)
Regarding cloning of Nus His tag fusion construct, to express double tagged fusion protein ? | ResearchGate
![SOLVED: Based on our vector we will need the Xhol and Ndel recognition sequence QQuestion 2 His:Tag Research the sequence for the Ndel cut site: Type your response here Xhol Ndel pET-28a SOLVED: Based on our vector we will need the Xhol and Ndel recognition sequence QQuestion 2 His:Tag Research the sequence for the Ndel cut site: Type your response here Xhol Ndel pET-28a](https://cdn.numerade.com/ask_images/5a4ffabce02148148b3f734b19046440.jpg)
SOLVED: Based on our vector we will need the Xhol and Ndel recognition sequence QQuestion 2 His:Tag Research the sequence for the Ndel cut site: Type your response here Xhol Ndel pET-28a
![Sequences of primers used for amplification of LIC cassette containing... | Download Scientific Diagram Sequences of primers used for amplification of LIC cassette containing... | Download Scientific Diagram](https://www.researchgate.net/publication/236910274/figure/fig8/AS:339883232120839@1458045885250/Sequences-of-primers-used-for-amplification-of-LIC-cassette-containing-start-codon.png)
Sequences of primers used for amplification of LIC cassette containing... | Download Scientific Diagram
![Cloning strategy, production and purification of proteins with exopeptidase-cleavable His-tags | Nature Protocols Cloning strategy, production and purification of proteins with exopeptidase-cleavable His-tags | Nature Protocols](https://media.springernature.com/full/springer-static/image/art%3A10.1038%2Fnprot.2006.388/MediaObjects/41596_2006_Article_BFnprot2006388_Fig1_HTML.jpg)