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Plants | Free Full-Text | Construction of Multiple Guide RNAs in CRISPR/Cas9 Vector Using Stepwise or Simultaneous Golden Gate Cloning: Case Study for Targeting the FAD2 and FATB Multigene in Soybean
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Cloning strategy to insert gRNAs into the pU6-BbsI-gRNA plasmid (A) The... | Download Scientific Diagram
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The PNA-induced DNA nicking by BbsI. ( a ) The DNA construct used in... | Download Scientific Diagram
![Plants | Free Full-Text | Construction of Multiple Guide RNAs in CRISPR/Cas9 Vector Using Stepwise or Simultaneous Golden Gate Cloning: Case Study for Targeting the FAD2 and FATB Multigene in Soybean Plants | Free Full-Text | Construction of Multiple Guide RNAs in CRISPR/Cas9 Vector Using Stepwise or Simultaneous Golden Gate Cloning: Case Study for Targeting the FAD2 and FATB Multigene in Soybean](https://www.mdpi.com/plants/plants-10-02542/article_deploy/html/images/plants-10-02542-g002.png)
Plants | Free Full-Text | Construction of Multiple Guide RNAs in CRISPR/Cas9 Vector Using Stepwise or Simultaneous Golden Gate Cloning: Case Study for Targeting the FAD2 and FATB Multigene in Soybean
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