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Plants | Free Full-Text | Construction of Multiple Guide RNAs in  CRISPR/Cas9 Vector Using Stepwise or Simultaneous Golden Gate Cloning: Case  Study for Targeting the FAD2 and FATB Multigene in Soybean
Plants | Free Full-Text | Construction of Multiple Guide RNAs in CRISPR/Cas9 Vector Using Stepwise or Simultaneous Golden Gate Cloning: Case Study for Targeting the FAD2 and FATB Multigene in Soybean

Team:Freiburg/Project/Golden - 2012.igem.org
Team:Freiburg/Project/Golden - 2012.igem.org

BbsI Restriction Enzyme - Laboratory Notes
BbsI Restriction Enzyme - Laboratory Notes

Strategy for the construction of the site-speci fi c, modi fi ed... |  Download Scientific Diagram
Strategy for the construction of the site-speci fi c, modi fi ed... | Download Scientific Diagram

Addgene: pKLV2.2-mU6gRNA5(SapI)-hU6gRNA5(BbsI)-PGKpuroBFP-W
Addgene: pKLV2.2-mU6gRNA5(SapI)-hU6gRNA5(BbsI)-PGKpuroBFP-W

Addgene: gRNA_Cloning Vector BbsI Sequences
Addgene: gRNA_Cloning Vector BbsI Sequences

BpiI (BbsI) (10 U/µl)
BpiI (BbsI) (10 U/µl)

Cloning strategy to insert gRNAs into the pU6-BbsI-gRNA plasmid (A) The...  | Download Scientific Diagram
Cloning strategy to insert gRNAs into the pU6-BbsI-gRNA plasmid (A) The... | Download Scientific Diagram

BbsI - BIOKÉ
BbsI - BIOKÉ

Part:BBa K1431201:Design - parts.igem.org
Part:BBa K1431201:Design - parts.igem.org

Addgene: pX330-BbsI-PITCh Sequences
Addgene: pX330-BbsI-PITCh Sequences

BbsI | NEB
BbsI | NEB

A User's Guide to Golden Gate Cloning Methods and Standards | ACS Synthetic  Biology
A User's Guide to Golden Gate Cloning Methods and Standards | ACS Synthetic Biology

BbsI | NEB
BbsI | NEB

Golden Gate Cloning - Wikipedia
Golden Gate Cloning - Wikipedia

Part:BBa K1431201:Design - parts.igem.org
Part:BBa K1431201:Design - parts.igem.org

The PNA-induced DNA nicking by BbsI. ( a ) The DNA construct used in... |  Download Scientific Diagram
The PNA-induced DNA nicking by BbsI. ( a ) The DNA construct used in... | Download Scientific Diagram

U6-gRNA (chiRNA) cloning – flyCRISPR
U6-gRNA (chiRNA) cloning – flyCRISPR

1 BBF RFC94 Type IIS Assembly for Bacterial Transcriptional Units
1 BBF RFC94 Type IIS Assembly for Bacterial Transcriptional Units

iLenti siRNA Cloning and Expression Vector (HIV based) (BbsI linearized)
iLenti siRNA Cloning and Expression Vector (HIV based) (BbsI linearized)

Plants | Free Full-Text | Construction of Multiple Guide RNAs in  CRISPR/Cas9 Vector Using Stepwise or Simultaneous Golden Gate Cloning: Case  Study for Targeting the FAD2 and FATB Multigene in Soybean
Plants | Free Full-Text | Construction of Multiple Guide RNAs in CRISPR/Cas9 Vector Using Stepwise or Simultaneous Golden Gate Cloning: Case Study for Targeting the FAD2 and FATB Multigene in Soybean

CRISPR-Cas9-mediated induction of large chromosomal inversions in human  bronchial epithelial cells - ScienceDirect
CRISPR-Cas9-mediated induction of large chromosomal inversions in human bronchial epithelial cells - ScienceDirect

Addgene: pU6-SacB-Cas9-T2A-mCherry_(BbsI) Sequences
Addgene: pU6-SacB-Cas9-T2A-mCherry_(BbsI) Sequences

gRNA cloning protocols – CRISPR Fly Design
gRNA cloning protocols – CRISPR Fly Design

BbsI | NEB
BbsI | NEB

Golden Gate Assembly - New England Biolabs GmbH
Golden Gate Assembly - New England Biolabs GmbH

1 BBF RFC94 Type IIS Assembly for Bacterial Transcriptional Units
1 BBF RFC94 Type IIS Assembly for Bacterial Transcriptional Units